MEKANISME ANTAGONIS LIMA ISOLAT Bacillus subtilis TERHADAP Colletotrichum capsici DAN C. gloeospoiroides IN VITRO

Nur Kholida Wulansari, Nur Prihatiningsih, Heru Adi Djatmiko

Abstract


Colletotrichum capsici dan C. gloeospoiroides adalah jamur patogen penting pada cabai merah yang dapat
menurunkan produktivitas. Upaya untuk mengatasi permasalahan ini yaitu dengan pengendalian hayati
menggunakan Bacillus subtilis. Penelitian ini dilakukan untuk mengetahui daya hambat lima isolat B.subtilis yaitu
B46, B209, B211, B298, dan B315 terhadap patogen C. capsici dan C. gloeospoiroides asal tanaman cabai.
Percobaan laboratorium dilakukan berdasarkan Rancangan Acak Lengkap dengan 5 ulangan. Pengujian in vitro
dilakukan menggunakan teknik dual culture pada medium PDA. Hasil penelitian menunjukkan efektifitas
penghambatan C.capsici paling baik pada isolat B209 sebesar 34,25%. Efektifitas penghambatan C.
gloeospoiroides pada isolat B211 sebesar 28,89%. Efektifitas penghambatan berpengaruh pada bobot kering
misellium dan morfologi hifa C. capsici dan C. gloeospoiroides. Morfologi hifa C. capsici dan C. gloeospoiroides
mengalami lisis, menebal, dan membengkak.
Kata kunci: C. capsici, C. gloeospoiroides, B. subtilis, antagonistik, daya hambat
ABSTRACT
Colletotrichum capsici and C. gloeospoiroides are an important pathogen on red chili pepper and caused
productivity losses. Biological control using B. subtilis is an attempt to solve the problem. The objectives of this
study is to determines the inhibition ability of five B. subtilis isolates, i.e. B46, B209, B211, B298, dan B315
against C. capsici and C. gloeospoiroides pathogens from chili pepper. Laboratory experinments arranged in
complete randomized design with five replication. Dual culture method used PDA medium on in vitro test. Result
showed the best inhibition effectiveness of C.capsici on B209 isolates at 34.25%. The best inhibition effectiveness
of C. gloeospoiroides on B211 isolates at 28,89%. The inhibition effectiveness affects mycellium dry weight and
hypha morphology of C. capsici and C. gloeospoiroides. Hypha morphology of C. capsici and C. gloeospoiroides
is lysis, thickening, and swelling.
Key words: C. capsici, C. gloeospoiroides, B. subtilis, antagonistic, inhibitor ability

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DOI: http://dx.doi.org/10.20884/1.agrin.2017.21.2.371

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Agriculture Research Journal
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