PENGARUH KONSENTRASI NATRIUM ALGINAT DAN JENIS BAL TERHADAP VIABILITAS SEL ENKAPSULASI PROBIOTIK BAL

Mukhtarudin Muchsiri, Basuni Hamzah, Agus Wijaya, Rindit Pambayun

Abstract

Tujuan penelitian ini untuk menguji pengaruh konsentrasi natrium alginat dan jenis BAL terhadap viabilitas
sel enkapsulasi probiotik BAL. Penyiapan enkapsulasi probiotik BAL menggunakan Rancangan Acak Kelompok
(RAK) faktorial, konsentrasi natrium alginat terdiri 1% (A1), 2% (A2) dan 3% (A3). Jenis BAL terdiri B1=
Lactobacillus bulgaricus, dan B2= Streptococus thermopylus dengan tiga ulangan. Parameter yang diamati terdiri
viabilitas sel BAL, jumlah enkapsul, massa enkapsul, bentuk dan ukuran enkapsul. Hasil penelitian menunjukkan
bahwa konsentrasi natrium alginat (w/v), jenis BAL, dan kelompok mempengaruhi nilai viabilitas sel BAL
digambarkan dengan persamaan Y = 10,753-0,235X1-1,1007X2+0,085X3+E. Rata-rata viabilitas tertinggi log 9,67
CFU/mL pada A3B1 (alginat 3% dan L. bulgaricus) dan terendah pada A1B2 (alginat 1% dan S. thermophylus) log
8,82 CFU/mL. Konsentrasi natrium alginat (w/v) dan jenis BAL mempengaruhi jumlah enkapsul digambarkan
dengan persamaan Y = 363,665-3,233X1+5,667X2+E. Rata-rata jumlah enkapsul tertinggi pada A3B1 (alginat 3%
dan L. bulgaricus) 375,33 enkapsul, dan terendah pada A1B2 (alginat 1% dan S. thermopylus) sebesar 360,67
enkapsul. Konsentrasi natrium alginat (w/v) dan jenis BAL mempengaruhi massa enkapsul digambarkan dengan
persamaan Y = 10,052-0,413X1+1,103X2+E. Rata-rata massa enkapsul tertinggi pada A2B1 (alginat 2% dan L.
bulgaricus) 12,659 gram, dan terendah pada A1B2 (alginat 1% dan S. thermopylus) sebesar 9,778 gram. Ukuran
diameter panjang enkapsul yang terpendek 7,5mm pada A3B2 (alginat 3% dan S. thermopylus) dan terpanjang
10mm pada A2B2 (alginat 2% dan S. thermopylus). Sedangkan diameter melintang berkisar 1,0 mm.
Kata kunci: enkapsulasi probiotik, BAL, viabilitas sel


ABSTRACT
The aim of this study were to examine the effect of sodium alginate concentration and types of LAB on cells
viability of LAB probiotic encapsulation. Preparation of LAB probiotic encapsulation using the randomized block
design (RBD) factorial, sodium alginate concentration (w/v) comprises 1% (A1), 2% (A2) and 3% (A3). The types
of LAB comprises Lactobacilus bulgaricus (B1), and Streptococous thermopylus (B2) with three replications. The
observed parameters consisted of cells viability, number, mass, type and size of bead from probiotics
encapsulation. The result showed that sodium alginate concentration (w/v) and types of LAB, block effect on cells
viability by the regression equation Y = 10,753-0,235X1-1,1007X2+0,085X3+E. The highest average viability log
9.67 CFU/mL on A3B1 (alginate 3% and L. bulgaricus) and the lowest in the A1B2 (alginate 1% and S.
thermophylus) log 8.82 CFU / mL. The sodium alginate concentration (w/v) and types of LAB effect on the amount
of probiotics encapsulation expressed by the regression equation Y = 363,665-3,233X1+5,667X2+E. The highest
average number of bead in A3B1 (alginate 3% and L. bulgaricus) 375.33 bead, and the lowest in A1B2 (alginate
1% and S. thermopylus) amounted to 360.67 bead. The sodium alginate concentration (w/v) and types of LAB
effect on mass of bead represented by the regression equation Y = 10,052-0,413X1+1,103X2+E. The highest
average mass of bead in A2B1 (alginate 2% and L. bulgaricus) 12.659 grams, and the lowest in A1B2 (alginate 1%
and S. thermopylus) amounted of 9.778 grams. While the size of the probiotics encapsulation diameter range from
the shortest length of 7.5 mm in A3B2 (concentration of 3% sodium alginate and S. thermopylus) and 10 mm in
the longest A2B2 (concentration of 2% sodium alginate and S. thermopylus), with a diameter ranging from 1.0 mm
Key words: Probiotics encapsulation, LAB, cells viability

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